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I, too, have been investigating Amazon EC2. I will be using Amazon’s S3 for a site, but have not yet determined if I want to also take advantage of Amazon’s Compute Cloud services. Either way, here are a few links that might point you in the right direction:

1. This guy is already doing what you want to do. He runs his WordPress-based site on EC2 using S3. He talks about the process in this article. Perhaps you could contact him for more details if needed.

This Web Site is Now Hosted on Amazon EC2 & S3

2. Information on using Amazon’s S3 with WordPress Mu:

– http://tantannoodles.com/toolkit/wordpress-s3/

– https://mu.wordpress.org/forums/topic.php?id=6351

– http://www.ringofblogs.com/2008/04/12/off-loading-wpmu-theme-files-to-amazon-s3/

Good luck! Let us know if you get this working.